Soapy layer on the top of the chloroform mixture!

[amroshk]

Hello, I have problem with DNA extraction. The problem arises after centrifuging the chloroform mixture with the WB, HB, etc, there is a green soapy layer on the top of the liquid sticks to the tip (I try to CTAB complexed proteins with chloroform). So, when I removing middle layer, the soapy layer stick to the tip and transfer to the new tube. Do you have any idea what does it happen? I followed all steps ignoring this problem and total DNA is quite good (enclosed gel). The only problem is that there is a big piece of garbage in the tube at the end that does not dissolve. Thanks a lot for your reply. Amroshk