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Soapy layer on the top of the chloroform mixture!

Soapy layer on the top of the chloroform mixture! - DNA Extraction Forum

Soapy layer on the top of the chloroform mixture! - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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Old 11-26-2010, 10:33 PM
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Default Soapy layer on the top of the chloroform mixture!



Hello, I have problem with DNA extraction. The problem arises after centrifuging the chloroform mixture with the WB, HB, etc, there is a green soapy layer on the top of the liquid sticks to the tip (I try to CTAB complexed proteins with chloroform). So, when I removing middle layer, the soapy layer stick to the tip and transfer to the new tube. Do you have any idea what does it happen? I followed all steps ignoring this problem and total DNA is quite good (enclosed gel). The only problem is that there is a big piece of garbage in the tube at the end that does not dissolve. Thanks a lot for your reply. Amroshk
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