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DNA extraction from bovine semen

DNA extraction from bovine semen - DNA Extraction Forum

DNA extraction from bovine semen - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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Old 09-21-2010, 09:32 AM
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Default DNA extraction from bovine semen



Can anyone help me with some protocol for extracting genomic DNA from bovine sperm, preferably without using Corex tubes.
Any help is appreciated.
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Old 09-22-2010, 03:20 PM
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Default Re: DNA extraction from bovine semen

Sperm are kind of funny, they resist SDS-Proteinase K. You need to treat them with DTT to break up their S-S bonds in the sperm cell coating.

Check out these links:
Differential extraction:
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