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Extraction from single fish egg

Extraction from single fish egg - DNA Extraction Forum

Extraction from single fish egg - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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Old 07-09-2010, 07:07 AM
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Default Extraction from a single fish egg



I will try to extract DNA from a single fish egg for pcr amplification and sequencing of the gene COI. I believe the egg should contain enough mitochondria to obtain a good sequence for this gene.

I'm planning to use Qiagen's spin column-procedure for the extraction.

The eggs were fixed and preserved in 96% ethanol. What is the optimal procedure for getting rid of the ethanol, drying the egg or placing the egg in water so the ethanol is diluted?

Feedback will be much appreciated.

Last edited by Audun; 07-09-2010 at 08:29 AM.
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Old 07-09-2010, 05:46 PM
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Default Re: Extraction from single fish egg

The alcohol fixation shouldn't be a problem, I used to do fish/shrimp disease PCR diagnostics with alcohol fixed tissue. I incubated 3mm^2 pieces of tissue for 20minutes in MilliQ water (~30-50mL) to dilute out the ethanol.
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