I am a first time user of PicoGreen (Quant-IT from Invitrogen)
and I need some answers...
1) Is it possible to quantitate DNA using PicoGreen on the LightCycler 480?
I want to prepare a microplate of samples, pop it into the LightCycler, draw up my standard curve, and quantitate dsDNA. Easy, right? Any ideas about setting up a protocol? I have contacted all reps... no-one seem keen to figure it out for me! I do know there are papers out there who have done it, but no details in the paper!! I am probably going to try mailing the authors too.
2) Can you quantitate PCR products
using PicoGreen? How would I adapt my protocol? I presume I would have to clean up my PCR products first? What would I use as standards though?