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DNA quantitation

DNA quantitation - DNA Extraction Forum

DNA quantitation - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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Old 06-30-2010, 10:30 AM
Pipette Filler
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Default DNA quantitation



Hi all,

I am a first time user of PicoGreen (Quant-IT from Invitrogen) and I need some answers...

1) Is it possible to quantitate DNA using PicoGreen on the LightCycler 480? I want to prepare a microplate of samples, pop it into the LightCycler, draw up my standard curve, and quantitate dsDNA. Easy, right? Any ideas about setting up a protocol? I have contacted all reps... no-one seem keen to figure it out for me! I do know there are papers out there who have done it, but no details in the paper!! I am probably going to try mailing the authors too.

2) Can you quantitate PCR products using PicoGreen? How would I adapt my protocol? I presume I would have to clean up my PCR products first? What would I use as standards though?

Thanks!

JM
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Old 04-02-2014, 11:13 AM
Pipette Filler
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Smile Re: DNA quantitation

Hi, did you suceed in setting up the quantitation - if so, I would like to hear the protocol!?
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