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-   -   Separation of Prokaryotic and Eukaryotic DNA ? (http://www.molecularstation.com/forum/dna-extraction-forum/72852-separation-prokaryotic-eukaryotic-dna.html)

wzacs1 04-24-2010 01:31 AM

Separation of Prokaryotic and Eukaryotic DNA ?
 
Does anyone have any ideas on how one could separate (or at least enrich) for bacterial DNA in a mixed sample of eukaryotic and bacterial DNA? Are there any restriction enzymes that will specifically degrade CpG methylated DNA for example? I am having a difficult time finding a method to do this. It must be specific. I want as much of bacterial DNA as possible and the eukaryotic DNA is totally worthless to me (except my own - I need that). Any help would be much appreciated! Thanks.

Tigerlilly 11-27-2011 10:29 PM

Re: Separation of Prokaryotic and Eukaryotic DNA ?
 
Hey there,

I have a quiet similar problem. I have been searching for several techniques and have tried different approaches. Did you find an appropriate protocoll to separate bacterial and human DNA? I would be very pleased if you could give me some hints or publications that my help me to solve the problem.

Many thanks in advance!


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