Separation of Prokaryotic and Eukaryotic DNA ?
Does anyone have any ideas on how one could separate (or at least enrich) for bacterial DNA in a mixed sample of eukaryotic and bacterial DNA? Are there any restriction enzymes that will specifically degrade CpG methylated DNA for example? I am having a difficult time finding a method to do this. It must be specific. I want as much of bacterial DNA as possible and the eukaryotic DNA is totally worthless to me (except my own - I need that). Any help would be much appreciated! Thanks.
Re: Separation of Prokaryotic and Eukaryotic DNA ?
I have a quiet similar problem. I have been searching for several techniques and have tried different approaches. Did you find an appropriate protocoll to separate bacterial and human DNA? I would be very pleased if you could give me some hints or publications that my help me to solve the problem.
Many thanks in advance!
|All times are GMT. The time now is 04:42 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved