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Tris base or tris buffer
We are currently trying to extract genomic DNA, but we are geting no result... What i think is their is some problem in the reagent prepartion, I am bit confused about equilibrated phenol prepartion , are we supposed to use Tris base or tris buffer in TrisCl production? What's the diff between them?
What must be the Ph of equilbrated phenol in order to get good DNA..?
|base , buffer , tris|
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