Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques > DNA Extraction Forum
Register Search Today's Posts Mark Forums Read

DNA Extraction Forum DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


Tris base or tris buffer

Tris base or tris buffer - DNA Extraction Forum

Tris base or tris buffer - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-10-2009, 03:04 AM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2009
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Tris base or tris buffer



Hi there,

We are currently trying to extract genomic DNA, but we are geting no result... What i think is their is some problem in the reagent prepartion, I am bit confused about equilibrated phenol prepartion , are we supposed to use Tris base or tris buffer in TrisCl production? What's the diff between them?


What must be the Ph of equilbrated phenol in order to get good DNA..?
Reply With Quote
Reply

Tags
base , buffer , tris


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
protein purification magnificientleo Proteomics Forum 11 12-29-2010 09:01 AM
SDS-PAGE Protocol moleculardude Protein Science 14 02-12-2010 11:27 AM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 2 01-08-2009 10:52 PM
Buffer Tris Question!!! please help! olenka Biochemistry Forum 2 09-18-2008 01:48 PM
TBS using Tris base vs Tris HCl Hannah2008 Western Blot Forum 0 08-04-2008 08:30 PM


All times are GMT. The time now is 03:55 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11348 seconds with 16 queries