I'm working on a project were I extract RNA from frozen breast tumortissue, using TriZOL for microarray analysis. I'm interestet in a protocol were I can extract DNA suitable for arrayCGH from the remaining lower/interphase.
I've done some testing were I've extracted DNA using the Back Extraction Buffer described by Ambions TRI-reagent and then done a clean-up using Qiagen qiaamp micro column. I manage to get 260/280 ratios around 1.8.
I also want to add a RNase step, but how and when do I do that?
Does anybody have experience with something similar og have any ideas to improve the protocol?