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adding RNase concentration?

adding RNase concentration? - DNA Extraction Forum

adding RNase concentration? - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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Old 06-29-2009, 05:30 AM
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Default adding RNase concentration?



what's the concentration to be used after extracting plasmid from cells?

20ug/ul and incubate for 20 minutes?

does adding higher concentration of RNase (eg 200ug/ul or 2mg/ul) have any side effects?
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Old 02-06-2012, 02:21 PM
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Default Re: adding RNase concentration?

I had the same question and what some people told me was that 20 g/ml is an usual concentration. Unfortunately, nobody was able to tell me sth about the side effects if the concentration in much higher. I guess it would interfere with the PCR reaction!?
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Old 02-12-2013, 03:09 PM
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