|Register||Search||Today's Posts||Mark Forums Read|
|DNA Extraction Forum DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.|
| ||LinkBack||Thread Tools||Display Modes|
adding RNase concentration?
what's the concentration to be used after extracting plasmid from cells?
20ug/ul and incubate for 20 minutes?
does adding higher concentration of RNase (eg 200ug/ul or 2mg/ul) have any side effects?
Re: adding RNase concentration?
I had the same question and what some people told me was that 20 µg/ml is an usual concentration. Unfortunately, nobody was able to tell me sth about the side effects if the concentration in much higher. I guess it would interfere with the PCR reaction!?
|adding , concentration , rnase|
|Thread||Thread Starter||Forum||Replies||Last Post|
|RNase A in Qiagen buffer P1||S. Schoenfeld||Protocols and Methods Forum||2||10-03-2012 03:40 PM|
|Measuring protein concentration after adding SDS sample buffer||deeps||Immunoprecipitation Forum||2||02-24-2011 05:32 AM|
|Low concentration miniprep||Utsugi, Heidi K||Protocols and Methods Forum||0||01-15-2009 05:16 PM|
|RNase away||Jayakumar, R||Protocols and Methods Forum||0||03-01-2006 02:49 AM|