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Getting low-yield with pGEX vector plasmids

Getting low-yield with pGEX vector plasmids - DNA Extraction Forum

Getting low-yield with pGEX vector plasmids - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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Old 09-08-2008, 04:17 PM
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Default Getting low-yield with pGEX vector plasmids



I have been trying to perform mini-preps w/ 5 ml of culture trying to isolate a group of pGEX plasmids. As well as doing this I have been running a positive control with a plasmids that I know works. Everytime I have performed this prep I've gotten very low yields while my positive control is still working. I was wondering if there could be an explanation as to the nature of the vector or possibly that my bacterial stocks in the -80 are just old. I've tried it with BL21 and TOP10 bacteria. Any suggestions would be appreciated thanks.
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dna , isolation , lowyield , mini , miniprep , pgex , plasmids , vector


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