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Linearized Vector DNA Purification TAE Agarose Gels

Linearized Vector DNA Purification TAE Agarose Gels - DNA Extraction Forum

Linearized Vector DNA Purification TAE Agarose Gels - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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Old 09-08-2008, 10:47 AM
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Default Linearized Vector DNA Purification TAE Agarose Gels



Hello research mates,
Im trying to isolate a 6kb plasmid vector that is linearized from restriction digestion.

The DNA was run on 0.8% TAE low melt agarose for gel purification. Im currently using the GFX PCR DNA Gel Band Purification kit (#27-9034-70) which melts the agarose slice and isolates it in spin columns.

I am eluting with autoclaved millipore water 30ul however it seems that i am loosing most (80%+) of my DNA!

is there any better way of purifying this DNA?
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agarose , dna , gels , linearized , purification , tae , vector


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