|Register||Search||Today's Posts||Mark Forums Read|
|DNA Extraction Forum DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.|
| ||LinkBack||Thread Tools||Display Modes|
Linearized Vector DNA Purification TAE Agarose Gels
Hello research mates,
Im trying to isolate a 6kb plasmid vector that is linearized from restriction digestion.
The DNA was run on 0.8% TAE low melt agarose for gel purification. Im currently using the GFX PCR DNA Gel Band Purification kit (#27-9034-70) which melts the agarose slice and isolates it in spin columns.
I am eluting with autoclaved millipore water 30ul however it seems that i am loosing most (80%+) of my DNA!
is there any better way of purifying this DNA?
|agarose , dna , gels , linearized , purification , tae , vector|
|Thread||Thread Starter||Forum||Replies||Last Post|
|agarose gel purification of cloning firstname.lastname@example.org||Protocols and Methods Forum||5||06-17-2010 09:33 AM|
|agarose gels||bahare||Agarose Gel Electrophoresis Forum||1||03-09-2010 05:29 PM|
|Sybr Green II and denaturing agarose gels||heliozoan||RNA Techniques Forum||2||05-11-2008 05:23 AM|
|migration of dyes and DNA thru alkaline agarose gels||Sharon||Protocols and Methods Forum||0||03-28-2008 03:36 AM|
|Agarose Gel Electrophoresis and DNA Fragment Purification||domba||Molecular Biology Articles and Protocols||0||07-31-2006 11:17 PM|