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-   -   Linearized Vector DNA Purification TAE Agarose Gels (http://www.molecularstation.com/forum/dna-extraction-forum/3904-linearized-vector-dna-purification-tae-agarose-gels.html)

Kellee 09-08-2008 10:47 AM

Linearized Vector DNA Purification TAE Agarose Gels
 
Hello research mates,
Im trying to isolate a 6kb plasmid vector that is linearized from restriction digestion.

The DNA was run on 0.8% TAE low melt agarose for gel purification. Im currently using the GFX PCR DNA Gel Band Purification kit (#27-9034-70) which melts the agarose slice and isolates it in spin columns.

I am eluting with autoclaved millipore water 30ul however it seems that i am loosing most (80%+) of my DNA! :gripe:

is there any better way of purifying this DNA?
:worshippy:


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