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Plasmid Extraction from Bacteria

Plasmid Extraction from Bacteria - DNA Extraction Forum

Plasmid Extraction from Bacteria - DNA Isolation Forum. A forum specifically about questions on DNA extraction and purification.


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  #1  
Old 09-01-2008, 05:25 AM
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Default Plasmid Extraction from Bacteria



Hi,
just received this question:

I'm currently doing a project on plasmids extraction
from bacteria.
The problem is I have been using a plasmid extraction kit, but
when I run an Ethidium Bromide electrophorese gel 1XTAE the results are
not satisfactory. I mean the is no clear fragments on the it always comes out
as smears.

Question is can you recommend a new protocol to follow please?
It will be highly appreciated.
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Old 06-28-2009, 06:02 AM
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Default Re: Plasmid Extraction from Bacteria

what protocol was he using? usually kits comes out nicely
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Old 06-29-2009, 01:45 AM
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Default Re: Plasmid Extraction from Bacteria

What protocol did you use? Did you use QIAGEN kits? It usually works well for me. If the gel shows smears, it is a sign of contamination or DNA degradation.
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