Originally Posted by admin
Make sure the tails are submerged in the buffer completely?
After proteinase K there should be only mouse hair no tail?
Did you vortex the lysed tail before adding them to the column?
Are you using proteinase K from the kit?
also too much tail 1.2 cm may not be good as it may be less digested and hence the column will have a problem with the collagen and tissue. How much tail does the kit suggest - 0.5cm or more / less?
the kit suggests 2 pieces of 1.2 cm mouse tail so i used the same.yes they are completely submerged as i cut them into smaller pieces before adding the buffer.after lysis there is only mouse hair (i think so) so i did a centrifugation step to remove the hair and used only the supernatant.
I vortex them well before adding to the spin coloumn and i am using the proteinase K from the kit.
yesterday with all these procedures in place the DNA concentration was 800ng/ul,the 260/280 ratio was 1.88.Is this good or can it be improved much more.