Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Confocal - Microscopy Imaging Techniques
Register Search Today's Posts Mark Forums Read

Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques

Microscope Design

Microscope Design - Confocal - Microscopy Imaging Techniques

Microscope Design - All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques

LinkBack Thread Tools Display Modes
Old 08-20-2013, 11:40 PM
Pipette Filler
Points: 34, Level: 1 Points: 34, Level: 1 Points: 34, Level: 1
Activity: 0% Activity: 0% Activity: 0%
Join Date: Aug 2013
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Microscope Design

Hello, I am currently an undergraduate working on a project with a few professors. I don't want to state to many specifics but I am worried that there may be some flaws in the confocal microscope design.

The design basically has four different lasers being sent parallel in the same direction down toward a target. Each laser has a beam expander added onto the front of it. Once the four lasers are directed parallel in the same line they are sent through an infinity lens system which consists of a 1st lens, pinhole, and 2nd lens. Although there is a beam expander added on to each laser, by the time they are directed toward the infinity lens system they are far smaller than necessary. The goal was to expand the laser beam diameter to fill an objective opening that was setup after the infinity lens system.

For now, to fix the issue, the 2 lenses used in the infinity lens system were changed to expand the beam to the necessary size. But, with that done, I see no point in expanding the original beams with beam expanders. I am not able to see any obvious flaws with the system but was curious if more experienced users of confocal microscope systems were able to point any faults out. So, my basic question is, does there need to be beam expanders on each of the lasers in the beginning? Why would the beam grow smaller?

Note: Two mirrors are used for the alignment of the laser. The mirrors are used to direct the beams toward the infinity lens system, then toward the objective.

Thank you for any input.
Reply With Quote

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Nikon Microscope - Eclipse 200 Best Clinical Scope? Microscope Co Lab Equipment Discussion and Reviews 1 04-18-2012 03:29 AM
Choosing A Microscope : Compound or Stereo microscope? nationalmicroscope Histology Forum 0 01-19-2010 08:11 AM
Need help with a microscope? ?Hollisterchikk16? Confocal - Microscopy Imaging Techniques 0 09-08-2009 02:16 AM
Scanning Microscope, Confocal Scanning Microscope and Transmission Electron... Mustafa M Confocal - Microscopy Imaging Techniques 1 08-06-2009 10:08 AM
ACS Short Course in Computational Chemistry and Drug Design Iosif Vaisman Protocols and Methods Forum 0 07-31-2005 04:00 AM

All times are GMT. The time now is 11:31 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11390 seconds with 16 queries