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Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


Rehydrating PFA-fixed cells?

Rehydrating PFA-fixed cells? - Confocal - Microscopy Imaging Techniques

Rehydrating PFA-fixed cells? - All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


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Old 12-20-2012, 09:52 PM
Pipette Filler
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Default Rehydrating PFA-fixed cells?



Hi,

I fixed some cultured epithelial cells to imaging plates for later immunostaining and analysis.

Protocol
1. Fixation in 4% PFA for 20 mins.
2. Rinse 3x with PBS.

I came back and found that I didn't seal the plate adequately and the PBS has evaporated. Can I rehydrate the PFA-fixed cells in PBS overnight and then immunostain the cells. Has anyone ever tried this before?

Thanks.
Silversickle
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