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Rehydrating PFA-fixed cells?
I fixed some cultured epithelial cells to imaging plates for later immunostaining and analysis.
1. Fixation in 4% PFA for 20 mins.
2. Rinse 3x with PBS.
I came back and found that I didn't seal the plate adequately and the PBS has evaporated. Can I rehydrate the PFA-fixed cells in PBS overnight and then immunostain the cells. Has anyone ever tried this before?
|cells , pfafixed , rehydrating|
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