Rehydrating PFA-fixed cells?
I fixed some cultured epithelial cells to imaging plates for later immunostaining and analysis.
1. Fixation in 4% PFA for 20 mins.
2. Rinse 3x with PBS.
I came back and found that I didn't seal the plate adequately and the PBS has evaporated. Can I rehydrate the PFA-fixed cells in PBS overnight and then immunostain the cells. Has anyone ever tried this before?
|All times are GMT. The time now is 11:52 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved