Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Confocal - Microscopy Imaging Techniques (http://www.molecularstation.com/forum/confocal-microscopy-imaging-techniques/)
-   -   Rehydrating PFA-fixed cells? (http://www.molecularstation.com/forum/confocal-microscopy-imaging-techniques/87014-rehydrating-pfa-fixed-cells.html)

silversickle 12-20-2012 09:52 PM

Rehydrating PFA-fixed cells?
 
Hi,

I fixed some cultured epithelial cells to imaging plates for later immunostaining and analysis.

Protocol
1. Fixation in 4% PFA for 20 mins.
2. Rinse 3x with PBS.

I came back and found that I didn't seal the plate adequately and the PBS has evaporated. Can I rehydrate the PFA-fixed cells in PBS overnight and then immunostain the cells. Has anyone ever tried this before?

Thanks.
Silversickle


All times are GMT. The time now is 04:35 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08834 seconds with 11 queries