Rehydrating PFA-fixed cells?
 

Hi,

I fixed some cultured epithelial cells to imaging plates for later immunostaining and analysis.

Protocol
1. Fixation in 4% PFA for 20 mins.
2. Rinse 3x with PBS.

I came back and found that I didn't seal the plate adequately and the PBS has evaporated. Can I rehydrate the PFA-fixed cells in PBS overnight and then immunostain the cells. Has anyone ever tried this before?

Thanks.
Silversickle