I made a construct with recA tagged with GFP under constitutive recA promoter on a plasmid in E coli.the cells are grown at 37C in lB with shaking at 150 rpm. When I view the cells in fluorecent microscope, I see green foci in ends of cells (which seem to me more like protein in inclusion bodies). however I also see the same foci fluorescing in CFP and YFP channel . I know that GFP and YFP spectra can overlap.. but CFP??? there is no CFP tagged protein in the cell.
Can any one suggest some remedy.
also what are the ways to prevent protein going into inclusion body?