Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Confocal - Microscopy Imaging Techniques
Register Search Today's Posts Mark Forums Read

Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


Help in Converting Stack to 3D image

Help in Converting Stack to 3D image - Confocal - Microscopy Imaging Techniques

Help in Converting Stack to 3D image - All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-04-2012, 12:31 AM
Pipette Filler
Points: 434, Level: 8 Points: 434, Level: 8 Points: 434, Level: 8
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Help in Converting Stack to 3D image



Hello Friends,

I have a small question and I would appreciate if you can help me in that. I have captured several z-stacks in olympus IX81. I want to convert them to 3D image. All I could do with the Metamorph software was conversion to 3D reconstruction, which was not too impressive.

Can you tell me with what software I can convert the stack to 3D image and how?

Thanks,

Vaibhav
Reply With Quote
  #2  
Old 02-29-2012, 12:23 PM
JWC JWC is offline
Pipette Filler
Points: 144, Level: 2 Points: 144, Level: 2 Points: 144, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2012
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Help in Converting Stack to 3D image

Hi,

I would recommend ImageJ. ImageJ is a free, open-source image manipulation package from the NIH and runs on any platform (Windows, Mac or Linux). Its functionality can be extended with plugins (see below).

Even more useful might be Fiji. As the name (Fiji Is Just ImageJ) suggests, it is simply ImageJ preloaded with large numbers of plugins, many of which are extremely useful for 3D reconstruction.

Either of these will let you register (align) the stacks, order them, crop them etc. They also do 3D-volume (but not, as far as I know, surface) reconstruction and can output a video of your 3D image slowly rotating and looking beautiful!

Hope that helps, there are many guides on how to use these packages out there - just google.

JWC
Reply With Quote
Reply

Tags
converting , image , stack


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Duck Bath Set Astrella@pasquale.com Forum De Chimie 0 05-21-2009 04:53 AM
Optoma Ezpro Projector Atthowe@lorna.com Forum Biologia 0 05-21-2009 02:53 AM
Biomedical Image Processing Call for Paper, Special Session within iCBBE2009 lmy liumeiy Conferences , Symposiums and Meetings 0 11-07-2008 03:51 PM


All times are GMT. The time now is 06:01 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11828 seconds with 16 queries