Help in Converting Stack to 3D image

[vab.patel]

Hello Friends,

I have a small question and I would appreciate if you can help me in that. I have captured several z-stacks in olympus IX81. I want to convert them to 3D image. All I could do with the Metamorph software was conversion to 3D reconstruction, which was not too impressive.

Can you tell me with what software I can convert the stack to 3D image and how?

Thanks,

Vaibhav

[JWC]

Hi,

I would recommend ImageJ. ImageJ is a free, open-source image manipulation package from the NIH and runs on any platform (Windows, Mac or Linux). Its functionality can be extended with plugins (see below).

Even more useful might be Fiji. As the name (Fiji Is Just ImageJ) suggests, it is simply ImageJ preloaded with large numbers of plugins, many of which are extremely useful for 3D reconstruction.

Either of these will let you register (align) the stacks, order them, crop them etc. They also do 3D-volume (but not, as far as I know, surface) reconstruction and can output a video of your 3D image slowly rotating and looking beautiful!

Hope that helps, there are many guides on how to use these packages out there - just google.

JWC