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mCherry expression in bacteria
I have mCherry alone and mCherry fused to my protein of interest in a arabinose inducible plasmid. In E. coli, I can only see fluorescence if cells are grown for at least 8h (to stationary phase) with 0.02% to 0.2% arabinose. I would like to see expression in exponential phase and think the expression should be evident 2 hours after adding inducer. Has anyone else run into this problem?
Thanks for your input in advance.
|bacteria , e. coli , expression , fluorescence , mcherry|
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