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Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


mCherry expression in bacteria

mCherry expression in bacteria - Confocal - Microscopy Imaging Techniques

mCherry expression in bacteria - All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


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Old 04-26-2011, 12:59 AM
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Question mCherry expression in bacteria



I have mCherry alone and mCherry fused to my protein of interest in a arabinose inducible plasmid. In E. coli, I can only see fluorescence if cells are grown for at least 8h (to stationary phase) with 0.02% to 0.2% arabinose. I would like to see expression in exponential phase and think the expression should be evident 2 hours after adding inducer. Has anyone else run into this problem?

Thanks for your input in advance.
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bacteria , e. coli , expression , fluorescence , mcherry


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