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-   -   mCherry expression in bacteria (http://www.molecularstation.com/forum/confocal-microscopy-imaging-techniques/82463-mcherry-expression-bacteria.html)

njs 04-26-2011 12:59 AM

mCherry expression in bacteria
 
I have mCherry alone and mCherry fused to my protein of interest in a arabinose inducible plasmid. In E. coli, I can only see fluorescence if cells are grown for at least 8h (to stationary phase) with 0.02% to 0.2% arabinose. I would like to see expression in exponential phase and think the expression should be evident 2 hours after adding inducer. Has anyone else run into this problem?

Thanks for your input in advance.


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