|Register||Search||Today's Posts||Mark Forums Read|
|Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques|
| ||LinkBack||Thread Tools||Display Modes|
Correct Immersion Oil
Hiya I am new to the world of confocal microscopy and am currently setting up an in house Nikon rig. I was wondering what would be the correct immersion oil(assuming matching index oil refraction to my 100X and 60X oil immersion objectives) Type DF(discontinued) or type A, or if type A can even be used at all. Any insight would be greatly appreciated!
Re: Correct Immersion Oil
What's critical is the refraction matching between your embedding medium, your slide and cover slips, and your immersion oil. For example, most borosilicate slides and glass cover slips have refraction index of 1.5 just as are the immersion oils. However, most embedding medium have very different refraction index. water embedded solution have a refraction index of 1.5. Electron Microscopy Science have a lot of different embedding media that are specific for different refractive index. I used CFM 1, a PBS-based mounting medium, which has an index very close to 1.5.
|correct , immersion , oil|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Is this statement about Active Transport correct?||tiffne11294||Biology Forum||0||11-12-2010 01:08 AM|
|what is the correct way to pronounce "apoptosis"?||Think.||Apoptosis, Autophagy, and Necrosis Forum||8||10-16-2008 03:21 AM|
|Oil Immersion Microscopy?||Brad S||Confocal - Microscopy Imaging Techniques||0||04-01-2007 05:55 PM|
|email@example.com , You have made comments that Momentum is not Conserved in my PAPER. It is not CORRECT. It is explained in 100 Years of E=mc2||AJAY SHARMA||Physics Forum||0||10-02-2006 12:59 PM|
|How do I use a partial immersion thermometer correctly?||AC/DCdude17||Chemistry Forum||1||12-15-2003 03:23 PM|