Correct Immersion Oil
 

Hiya I am new to the world of confocal microscopy and am currently setting up an in house Nikon rig. I was wondering what would be the correct immersion oil(assuming matching index oil refraction to my 100X and 60X oil immersion objectives) Type DF(discontinued) or type A, or if type A can even be used at all. Any insight would be greatly appreciated!

Re: Correct Immersion Oil
 

What's critical is the refraction matching between your embedding medium, your slide and cover slips, and your immersion oil. For example, most borosilicate slides and glass cover slips have refraction index of 1.5 just as are the immersion oils. However, most embedding medium have very different refraction index. water embedded solution have a refraction index of 1.5. Electron Microscopy Science have a lot of different embedding media that are specific for different refractive index. I used CFM 1, a PBS-based mounting medium, which has an index very close to 1.5.