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Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


Resolving power of a microscope question?

Resolving power of a microscope question? - Confocal - Microscopy Imaging Techniques

Resolving power of a microscope question? - All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


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Old 09-03-2009, 04:19 AM
Pipette Filler
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Default Resolving power of a microscope question?



I have homework due tomorrow and I did everything except this one question which I really need help with.

What 2 factors can affect the resolving power of a microscope?
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Old 09-17-2009, 02:55 PM
Pipette Filler
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Default Re: Resolving power of a microscope question?

RP = wavelength/2 NA

NA is the numerical aperture (inscribed on the barrel of the objective lens). It usually is between 0.4 (low power) and 1.25 (oil immersion).

Use the formula and you can see that the resolving power (RP) is dependent on wavelenght and numerical aperture.
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