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Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


In microscopy, which of the following combination gives you the best resolution?

In microscopy, which of the following combination gives you the best resolution? - Confocal - Microscopy Imaging Techniques

In microscopy, which of the following combination gives you the best resolution? - All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


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Old 06-21-2009, 05:16 PM
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Old 06-21-2009, 05:16 PM
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Default In microscopy, which of the following combination gives you the best resolution?

Hey guys!! I am in desperate need for help, I have taken this exam twice and looked over my answers. First got an 80 and then an 88, i can only take it once more and I really really NEED a solid A. I dont know what im doing wrong, if someone could help me, it would be highly appreciated. Thanks!

1.
In microscopy which of the following combination gives you the best resolution?
A) small numerical aperture lens and longer wavelength radiation.
B) Large numerical aperture and shorter wavelength radiation.
C) Large numerical aperture lens and longer wavelength radiation.
D) Small numerical aperture fore lens and radiation with shorter wavelength.

2.
The largest group of archaea is the
A) thermophiles.
B) halophiles.
C) cyanobacteria.
D) methanogens.

3.
Which of the following bacterial genera protects areas of the body such as the intestinal tract and the vagina from invasion by pathogens?
A) Listeria
B) Corynebacterium
C) Mycobacterium
D) Lactobacillus
E) Staphylococcus

4.
Which of the following types of microscopes is capable of generating a three-dimensional image of a specimen?
A) differential interference contrast
B) confocal
C) scanning electron
D) A, B, and C are correct.

5.
Which of the following is NOT true of transmission electron microscopy?
A) Electrons are used for magnification.
B) Glass lenses are used for refraction of electrons.
C) The specimen need to be very thin.
D) The sample is placed in a vacuum.

6.
All of the following are associated with clostridia EXCEPT:
A) botulism.
B) "flesh-eating" bacteria.
C) gangrene.
D) tetanus.

7.
The largest group of archaea is the
A) cyanobacteria.
B) halophiles.
C) thermophiles.
D) methanogens.
E) hyperthermophiles.

8.
Lipid A is also known as
A) teichoic acid.
B) pilin.
C) endotoxin.
D) mycolic acid.
E) lipopolysaccharide.

9.
Which of the following pairs is mismatched?
A) Lysosome protein synthesis
B) Mitochondria ATP production
C) Golgi complex packaging & secretion
D) Endoplasmic reticulum internal transport

10.
Which of the following chemical substances contributes to the unique characteristics of acid-fast bacteria?
A) mycolic acid
B) lipoteichoic acid
C) endotoxin
D) peptidoglycan
E) N-acetylglucosamine

11.
Which of the following types of microscopes is used for viewing biofilms?
A) brightfield
B) scanning electron
C) confocal
D) Both B and C are correct.
E) A, B, and C are correct.

12.
Which of the following lense (s) is/are involved in total magnification of a specimen?
A) objective
B) ocular
C) condenser
D) A and B are correct.
E) A, B, and C are correct.

13.
Which of the following groups of bacteria live in environments similar to those that may have existed on the early Earth?
A) deeply branching bacteria
B) proteobacteria
C) cyanobacteria
D) thermophiles

14.
When biologists wish to study the internal ultrastructure of cells, they most likely would use
A) Scanning tunneling microscope
B) a good light microscope
C) a scanning electron microscope
D) a transmission electron microscope

15.
Which of the following types of microscopes gives you the highest magnification?
A) a dark-field microscope.
B) a scanning tunneling microscope.
C) a fluorescent microscope.
D) a differential interference contrast microscope.
E) a phase-contrast microscope.

16.
Which among the following achieves the highest magnification and greatest resolution.
A) Compound light microscope
B) Phase-contrast microscope
C) Darkfield microscope
D) Fluorescence microscope
E) Electron microscope

17.
The appearance of gram-negative bacteria after addition of the decolorizing agent in the Gram stain.
A) Purple
B) Red
C) Colorless
D) Brown
E) None of the above

18.
Which of the following organelles most closely resembles a prokaryotic cell?
A) Golgi complex
B) Mitochondrion
C) Cell wall
D) Vacuole
E) Nucleus

19.
The purpose of a mordant in the Gram stain is
A) To prevent the crystal violet from leaving the cells.
B) To make the bacterial cells larger.
C) To remove the simple stain.
D) To make the flagella visible.
E) None of the above.

20.
Which of the following statements concerning prokaryotic flagella is false?
A) Treponema is an example of a bacterium that has an endoflagellum.
B) Prokaryotic flagella are composed of tubulin.
C) Prokaryotic flagella are anchored to the cell wall by means of the basal body.
D) Prokaryotic flagella can rotate 360 degrees.

21.
Flesh eating bacteria belong to the genus
A) Salmonella.
B) Staphylococcus.
C) Pseudomonas.
D) Streptococcus.

22.
Which of the following is generally not true of prokaryotic cells?
A) They are motile by means of flagella.
B) Their DNA is located in the nucleoid region
C) They possess 80S ribosomes.
D) They have a cell wall.
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