| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| In biology, just say you had a cell and wanted to view it under the microscope, why do you put water on the slide before placing he coverslide on top? Thanks in advance! |
|
#2
| |||||||||||
| |||||||||||
| hi Many objects that are going to be viewed on a compound light microscope slide are prepared as a wet mount using water. Other materials are used when a permanent slide is being prepared for viewing and storage. In a wet mount, the specimen is placed at the center of the slide with one or two drops of water and the cover glass placed over the specimen. In some preparations (such as looking at pond water for microscopic critters), the object being prepared for viewing is contained within water. Special slides are available for viewing mounts that require more than one or two drops of water. Once the specimen and water are combined on the slide, the cover glass is added. The cover glass should be placed at an angle to the slide, one edge touching the slide, and then lowered as if hinged there. If done properly, the water will force out any air as the cover glass closes over it, and no bubbles will be trapped beneath the glass. Although an occasional bubble might be tolerated, large numbers will make viewing the specimen difficult. Adhesive forces between the liquid and the glass will hold the cover glass firmly in place. Generally, only one drop of water is sufficient. Adding too much water will create a problem, as the affixing of the cover slip to the slide will depend on much weaker cohesive forces (see "Problems and solutions" below). There should be no excess water outside the cover slip, and the cover slip should remain in place when the slide is moved to the stage of the microscope, where it is held in place by stage clips or a mechanical stage arm. |
|
#3
| |||||||||||
| |||||||||||
| it acts as a glue. It sorta holds stuff in place and suctions the slides together. |
|
#4
| |||||||||||
| |||||||||||
| So nothing moves underneath it. The water sort of sucks down the cover slide and helps it to stay in place. |
|
#5
| |||||||||||
| |||||||||||
| because it would be hard to focus if you didn't do that |
|
#6
| |||||||||||
| |||||||||||
| Wet mounting requires water. It is a slide preparation technique that is commonly used to contain living organisms, whether or not they are mobile. Preparing the slide on a wet mount basically follows the same procedure as the dry mount technique. The only difference is that water is being added to the equation. |
|
#7
| |||||||||||
| |||||||||||
| Otherwise the cell would not be visible through all that distracting air. And it would also be dehydrated and scrunched up! |
|
#8
| |||||||||||
| |||||||||||
| Im not sure but I think the vision is magnified with the water, kind of like how things look bigger underwater |
| Tags |
| microscope , microscopic , put , slides , viewing , water , _ |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Repeat performance to the tee;Safeway Water contaminated? Coliform bacteria? Same as 2003 incidentposted! Have specimen in Chicago | Kevin Dixler | Microbiology Forum | 1 | 10-16-2007 01:22 AM |
| Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! | jon_johnfrancisayres@yahoo.com | Microbiology Forum | 0 | 10-06-2007 05:59 AM |
| GNU units and units.dat; Units of Measurement and Unit Conversion | James Redford | Physics Forum | 0 | 07-31-2005 12:08 PM |
| Sci.chem FAQ - Part 6 of 7 | Bruce Hamilton | Chemistry Forum | 0 | 01-15-2004 08:12 AM |