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What is the highest magnification a light microscope is capable of?

What is the highest magnification a light microscope is capable of? - Confocal - Microscopy Imaging Techniques

What is the highest magnification a light microscope is capable of? - All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


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Old 06-10-2009, 04:18 AM
Pipette Filler
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Default What is the highest magnification a light microscope is capable of?



Not size or wavelength, magnification.
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Old 06-10-2009, 04:18 AM
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im guessing on this but if you could power it and had the right technoligy then probably as much as you would want
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Old 06-20-2009, 01:02 AM
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Default Re: What is the highest magnification a light microscope is capable of?

Quote:
Originally Posted by guderian93 View Post
Not size or wavelength, magnification.

Typically, the highest objective you will encounter on a light microscope is 100x oil.

Theoretically, if you have 20x eyepieces, you could achieve 2000 magnification.

In reality, you begin to lose resolution beyond 1000x magnification on a light microscope. Thus, the standard combination on research light microscopes is 10x eyepieces with a 100x oil objective and a numerical aperture of 1.4.


The law of diminishing returns applies to your question.

Optimal viewing with a light microscope for maximum magnification occurs at 1000x via the use of 10x eyepieces and a 100x oil objective with a 1.4 numerical aperture when used with the proper oil application.

Anything beyond that starts to sacrifice resolution and optical clarity for sheer numbers.

I hope that helps you have a better understanding.
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