Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Confocal - Microscopy Imaging Techniques
Register Search Today's Posts Mark Forums Read

Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


Confocal Microscopy Method

Confocal Microscopy Method - Confocal - Microscopy Imaging Techniques

Confocal Microscopy Method - All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-06-2006, 07:56 PM
admin's Avatar
Administrator
 
Join Date: Nov 2005
Posts: 1,418
Thanks: 883
Thanked 68 Times in 58 Posts
Arrow Confocal Microscopy Method



Hi there everyone!

is anyone here an expert on confocal microscopy?

if you have even worked with it once, please post here your experiences.

thanks

Admin
Reply With Quote
  #2  
Old 11-06-2006, 10:06 PM
Graduate Student
Points: 2,923, Level: 35 Points: 2,923, Level: 35 Points: 2,923, Level: 35
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2006
Location: Ithaca, NY
Posts: 145
Thanks: 0
Thanked 17 Times in 12 Posts
Default Re: Confocal Microscopy Method

You'd probably get more people finding the Confocal forum if its name weren't misspelled.

Confocal microscopy in and of itself is uninteresting. What's interesting is what you do with it, and that's hard to standardize. It's quite different looking at a fixed cell than a live one, for instance, or visualizing protein localization by GFP or immunofluorescence versus localizing chromosomes and genes by FISH, versus measuring nuclear NAD levels by autofluorescence.

I guess if I understood what your goals were for this post, it would make more sense to me.
Reply With Quote
  #3  
Old 11-07-2006, 01:09 AM
admin's Avatar
Administrator
 
Join Date: Nov 2005
Posts: 1,418
Thanks: 883
Thanked 68 Times in 58 Posts
Default Re: Confocal Microscopy Method

Hey Kmunson,
thanks for spotting that!

Yes, I agree its a general term but I am hoping people with microscopy experience can share their insight into the technique and the methodology of it.
Reply With Quote
  #4  
Old 03-23-2007, 11:14 PM
Pipette Filler
Points: 1,355, Level: 21 Points: 1,355, Level: 21 Points: 1,355, Level: 21
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2007
Posts: 9
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Confocal Microscopy Method

Hi guys,

I would say that I have a good amount of experience with confocal microscopy. I was working with many confocals using many different techniques.
But as it was already posted here: It is better to answer specific questions rather than giving an oversight what you can do with it or how you do it
Reply With Quote
  #5  
Old 10-07-2007, 02:21 AM
Pipette Filler
Points: 2,187, Level: 29 Points: 2,187, Level: 29 Points: 2,187, Level: 29
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2007
Posts: 16
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Confocal Microscopy Method

HI there,

confocal microscopy is great if you want to scan a tissue that is thicker than a 6-8um microscope slide. It is great to scan different layers of a tissue, for example, using murine corneas, you can scan from the apical epithelial layers to the basal and even to the stroma cells and even endothelium cells. It all depends on your intention and time. Usually confocal microscopy can be very long.

I hope that helps.
Reply With Quote
  #6  
Old 01-26-2008, 07:04 AM
Pipette Filler
Points: 766, Level: 15 Points: 766, Level: 15 Points: 766, Level: 15
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2008
Location: assam,nalbari
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Confocal Microscopy Method

u r right, i do need a help in understanding working with confocal microscopy..interested and experienced lots are invited to post their views..
Reply With Quote
  #7  
Old 04-24-2008, 11:08 AM
Pipette Filler
Points: 553, Level: 11 Points: 553, Level: 11 Points: 553, Level: 11
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2008
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Confocal Microscopy Method

I am working in the cell culture area. I am trying to do some cell staining/immunofluorescence by growing cells in cover slips (very problematic - with breakages). I have 2 primary antibodies.. anti-hexokinase 2 and Hsp 60 which is a mitochnodrial marker, we want to conjugate with secondary fluoresecent ab and see if there is any co-localisation.

What advice can you give me interms of a btter or more stringent protocol to ensure I get the images i'm looking for?

1. one important question on my bind is whether or not I should incubate with both primary anibodies simulataneously or in step-wise fashion?

Many thanks

playerJS
Reply With Quote
  #8  
Old 04-25-2008, 08:54 AM
Pipette Filler
Points: 560, Level: 11 Points: 560, Level: 11 Points: 560, Level: 11
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2008
Posts: 10
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Confocal Microscopy Method

I have been working with it for quite some time and use it to visulise FPs in plant tissue. Did you have any specific questions?

I think it is a vital part to my research.
Reply With Quote
  #9  
Old 11-09-2008, 03:56 PM
Pipette Filler
Points: 175, Level: 3 Points: 175, Level: 3 Points: 175, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2008
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Confocal Microscopy Method

We have just acquired an Olympus FV-1000 and would like to show students some quick and easy results. To avoid fixing and permeabilizing, I would like to ask if users have recommendations for good vital stains.
Thanks
Reply With Quote
Reply

Tags
confocal , method , microscopy


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Confocal Microscopy Exam Question -- Please Help travisjbamford Confocal - Microscopy Imaging Techniques 0 04-24-2010 03:53 PM
Human Cytome Project - How to explore - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 0 04-19-2005 07:10 AM
Human Cytome Project - How to Explore - Update 14 March 2005 Peter Van Osta Cell Biology and Cell Culture 0 03-14-2005 02:31 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 05:56 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.19909 seconds with 16 queries