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Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


VSVG-GFP fixed slide prep

VSVG-GFP fixed slide prep - Confocal - Microscopy Imaging Techniques

VSVG-GFP fixed slide prep - All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques


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Old 08-03-2008, 12:36 PM
Pipette Filler
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Default VSVG-GFP fixed slide prep



If I am given a fixed slide prep of VSVG-GFP transfected cells, what method can I use to tell if the fluorescent cargo protein is co-localized with the coat protein (Rab1 I think)? What reagent(s) will I need?
Thanks for your help.
D.
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Old 08-05-2008, 04:46 PM
Pipette Filler
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Default Re: VSVG-GFP fixed slide prep

You will likely need to label the slide with the Rab1 protein using a primary antibody against it (and then washing, applying a secondary antibody conjugated to a fluorescent dye that is not green, and then washing, and then adding mounting solution). You should then get some training on using a fluorescence microscope (confocal would probably be better), and check for co-localization of the two proteins after you merge the two images together once you have taken a picture of the localization of each of the GFP and the Rab1 protein.
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