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| Confocal - Microscopy Imaging Techniques All about Microscopy, Confocal Microscopy, Fluorescence microscopy and Other Imaging Techniques |
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| When you look into the microscope, did the field (and thus the specimen) seem to be as brightly illuminated when the higher power objectives were used? how might (or did) you correct for this change in illumination? |
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| It depends on the type of 'scope you are using. Some are brighter, some are the same, and others are not as bright. The way to correct it is by opening or closing the iris diaphragm. This lets in more light, or less, depending if you open or close it. Another way, if your 'scope is without a diaphragm is by using the condenser. This will do the same as the iris, sort of, it projects a larger or smaller amount of light precisely under your specimen, whereas the diaphragm lets light on throughout the entire area under the slide. |
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