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| Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques. |
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| wHEN I ANALYSE PARABENS AND PHENOXYETHANOL IN A C18 COLUMN, USING METANOL/H2O (38:62) AS MOBILE PHASE, WITH A NEW COLUMN I SEPARATE THEM VERY GOOD. BUT IN A SECOND ANALYSE I HAVE A LOT OF PROBLEMS IN GET ISOMETRIC PICS, INSTEAT OF PICS THEY SEEM TO BE MOUNTAINS.. WHAT HAVE HAPENNED? HAS THE STATIONARY PHASE BEEN DAMAGED? WHY? HOW DO I HAVE TO KEEP MY COLUMN WHEN IT HASN'T BEEN USED? |
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