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Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


HPLC Validation - Calibration Curve

HPLC Validation - Calibration Curve - Chromatography Forum

HPLC Validation - Calibration Curve - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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Old 09-02-2013, 02:33 PM
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Default HPLC Validation - Calibration Curve



Hi Guys

I am currently developing and validating an ion chromatography method in my laboratory. This is my first time performing a proper validation and I have a question regarding calibration.

From the guidelines I have been following to perform my validation, it states that 'the blank matrix sample should not be taken into consideration when plotting calibration curves'. I was wondering wether this approach was taken by others who have previous experience of method validation? How do you construct your graph i.e. force point through zero? plot blank sample? only plot validation standards minus the blank sample?

Thank you for your help in advance.
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