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-   -   HPLC troubleshoot (http://www.molecularstation.com/forum/chromatography-forum/84345-hplc-troubleshoot.html)

dinesh2dg 12-20-2011 12:49 PM

HPLC troubleshoot
 
Hi all,
I was using RP HPLC for carbohydrates separation. I used to get a solvent peak ahead of the analytes. Now, all of a sudden, the solvent peak got inversed. I dont know why. Can someone help me out of this.

Thanks in advance.

dinesh2dg 12-20-2011 01:19 PM

Re: HPLC troubleshoot
 
Please read inverse peaks as Negative peak.

vulcanian 04-08-2012 05:29 PM

Re: HPLC troubleshoot
 
carbohydrates separation? witch is your detector? RI detection? the solvent peak may be due to the injection sample is different (different solvent mixture), if the sample is prepared in other condition that could be OK but it is always a good idea to dope it to control that everything is OK.

beware also negative peaks in my experience are usually related to bubles or problems with the detector lamp (UV detectors) but when that happens is phenomen along all the chromatogram not only for the injection peak

Hotsummerly 07-13-2013 02:30 AM

Re: HPLC troubleshoot
 
I am interested in the Explorer 100 - What is the version of Unicorn?
Thank you.


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