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HPLC column clogging

HPLC column clogging - Chromatography Forum

HPLC column clogging - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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Old 10-08-2010, 12:31 PM
Pipette Filler
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Default HPLC column clogging



Hai everyone,
What solvent would help me to get rid of column clogs when even methanol failed to solve the problem. Or any method to get rid of clogging of HPLC columns
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Old 10-20-2010, 06:49 PM
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Default Re: HPLC column clogging

Hello,
What type of column is it?
When I have this problem, I will first try to clean with IPA (at ~.3mL/min) overnight. Disconnect the column outlet from your detector to ensure that you don't end up contaminating the detector.
Most modern columns can be washed backwards to get rid of particles at the head of the column. I do this with a low flow of IPA as well. You may want to call your column manufacturer to ensure that its okay to do this with your column type.
Last resort, Dichloromethane.

In the future, use a guard column if you can. They are much cheaper to throw away if they can't be cleaned.

Lisa
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