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Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


FPLC set up

FPLC set up - Chromatography Forum

FPLC set up - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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Old 08-16-2010, 07:55 PM
Pipette Filler
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Default FPLC set up



I just inherited an old Pharmacia FPLC --LCC-500, 2 P-500 pumps, MV-7 injection valves, and fraction collector. I need to clean up some peptides via RP so it would be great if I could get it working. Everything turns on and seems OK except I always get error code saying pumps are busy(LCC-500 error codes 6,7). If I hold the run button down, the red light goes on, no error code, but the button will not stay in the run (depressed) position(so I let go and the error and beeping starts again, angering other lab members). I fear I am doing something stupid, or missing a basic feature of the system but really need this. Does anyone have experience with this system and know what might be the problem. Thanks
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