Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Chromatography Forum (http://www.molecularstation.com/forum/chromatography-forum/)
-   -   FPLC set up (http://www.molecularstation.com/forum/chromatography-forum/73585-fplc-set-up.html)

Timothyc29 08-16-2010 07:55 PM

FPLC set up
 
I just inherited an old Pharmacia FPLC --LCC-500, 2 P-500 pumps, MV-7 injection valves, and fraction collector. I need to clean up some peptides via RP so it would be great if I could get it working. Everything turns on and seems OK except I always get error code saying pumps are busy(LCC-500 error codes 6,7). If I hold the run button down, the red light goes on, no error code, but the button will not stay in the run (depressed) position(so I let go and the error and beeping starts again, angering other lab members). I fear I am doing something stupid, or missing a basic feature of the system but really need this. Does anyone have experience with this system and know what might be the problem. Thanks


All times are GMT. The time now is 11:11 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08814 seconds with 11 queries