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Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


determination of Vitamin A by HPLC

determination of Vitamin A by HPLC - Chromatography Forum

determination of Vitamin A by HPLC - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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Old 08-16-2010, 02:20 PM
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Default determination of Vitamin A by HPLC



Halo guys, i am recently working on the extraction of vitamin A in food. The standard preparation have to go through the extraction procedure like the sample prep. The standard solution(retinol palmitate) before go through the extraction has a peak at around 23 min,but for the standard that went through the extraction procedure, the peak no longer appear at 23min, it appear at around 3 min and got other small peaks after 3 min. May i know izit all the Retinol palmitate has already degraded through the process of extraction? It is reversed phase chromatography.

Need all ur helps...Thanks..
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