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Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


My Life is suppose to be easier.

My Life is suppose to be easier. - Chromatography Forum

My Life is suppose to be easier. - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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Old 06-11-2010, 12:27 PM
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Default My Life is suppose to be easier.



Hi, I was told by others that I was very lucky that I could use TLC to run activity assays on a protein whose structure we recently solved with NMR. TLC was suppose to be easier then our traditional approach of performing the assay on a large gel.

I'm having lots of problems getting readable results. Is there anyone who can suggest a good protocol or can provide any subtle hints to the technique.

The substrate is 5' labeled tRNAHis.

Thanks

Chris
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