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| Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques. |
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| Hi, I was told by others that I was very lucky that I could use TLC to run activity assays on a protein whose structure we recently solved with NMR. TLC was suppose to be easier then our traditional approach of performing the assay on a large gel. I'm having lots of problems getting readable results. Is there anyone who can suggest a good protocol or can provide any subtle hints to the technique. The substrate is 5' labeled tRNAHis. Thanks Chris |
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| easier , life , protein , protein assay , suppose , tlc |
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