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jerseysurfer66 06-11-2010 12:27 PM

My Life is suppose to be easier.
 
Hi, I was told by others that I was very lucky that I could use TLC to run activity assays on a protein whose structure we recently solved with NMR. TLC was suppose to be easier then our traditional approach of performing the assay on a large gel.

I'm having lots of problems getting readable results. Is there anyone who can suggest a good protocol or can provide any subtle hints to the technique.

The substrate is 5' labeled tRNAHis.

Thanks

Chris


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