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Unknown protein purification... need help

Unknown protein purification... need help - Chromatography Forum

Unknown protein purification... need help - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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Old 03-04-2010, 01:35 PM
Pipette Filler
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Default Unknown protein purification... need help



Hi,
I have one unknown protein of molecular weight 31 kDa. pI 8.2, stable at around pH 4- pH 10, expressed in soluble fraction at 10-12 mg per liter of original culture.
Thats all i have?
on the basis of molecular weight i think so i cant go for molecular sieve chromatography. But as it has pI of 8.2 and stable in acidic pH, I think so i should go for cation exchange chromatography.

Am i going rite?
and what does the lat part mean, expressed in soluble fraction at 10-12 mg per liter of original culture - --------> can we determine any technique from this information or its just for column capacity selection. If yes what should be the column capacity.
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