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#1
03-04-2010, 12:35 PM
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 Unknown protein purification... need help Hi, I have one unknown protein of molecular weight 31 kDa. pI 8.2, stable at around pH 4- pH 10, expressed in soluble fraction at 10-12 mg per liter of original culture. Thats all i have? on the basis of molecular weight i think so i cant go for molecular sieve chromatography. But as it has pI of 8.2 and stable in acidic pH, I think so i should go for cation exchange chromatography. Am i going rite? and what does the lat part mean, expressed in soluble fraction at 10-12 mg per liter of original culture - --------> can we determine any technique from this information or its just for column capacity selection. If yes what should be the column capacity.  |
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| protein , purification , unknown |
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