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Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


Need Help - losing resolution

Need Help - losing resolution - Chromatography Forum

Need Help - losing resolution - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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Old 04-02-2009, 01:41 PM
Pipette Filler
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Default Need Help - losing resolution



Problem: losing resolution in sample containing DPH and patent blue dye (liquigel contains form of bone & hide gelatin)
Conditions: Luna 2 C18, 4.6 x 150mm, mobile phase contains 65 water + .5mls triethylamine(TEA) pH to 6.2 w/ phosphoric acid:35 acetonitrile
at 220nm
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