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Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


HPLC analysis of sucrose

HPLC analysis of sucrose - Chromatography Forum

HPLC analysis of sucrose - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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  #1  
Old 01-31-2009, 02:06 PM
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Default HPLC analysis of sucrose



Hello, I'm trying to develop a method for sucrose analysis (just identity and not quantification) and I know reverse phase is not one of the options, so what is recommended; normal phase HPLC? and is it only refractive index detectors that can be used? no way to use UV?vis detector? Awaiting your help
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Old 12-01-2009, 02:18 PM
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Default Re: HPLC analysis of sucrose

thanks
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Old 12-03-2009, 05:45 PM
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Default Re: HPLC analysis of sucrose

Dionex Ion Chromotography with pH detection. CarboPac column. They should be able to help you. I used it for quantification, but identity is easier
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Old 12-16-2009, 06:32 AM
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Default Re: HPLC analysis of sucrose

very very interestating
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Old 12-16-2009, 06:35 AM
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Default Re: HPLC analysis of sucrose

very very good
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Old 08-10-2010, 09:50 AM
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Default Re: HPLC analysis of sucrose

I am having shimadzu Reverse Phase HPLC with RI detector and ODS C18 column. Can I estimate sucrose in peanut seeds with the above equipment. kindly give any protocol pertaining to this.
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Old 08-25-2010, 09:57 AM
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Default Re: HPLC analysis of sucrose

Hi,
In an HPLC analysis procedure, a small volume of the sample is introduced to the mobile phase stream; the pace of progress of the analyte through the column will be quicker or slower depending on the physical and/or chemical interactions between the analyte and the stationary phase as the sample travels through the column.

As stated above, the time between introduction of the sample and elution is referred to as the retention time; this time provides a relatively unique characteristic of the analyte which is useful in identification of the compounds contained in the sample.

Thanks.
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Old 12-20-2011, 12:40 PM
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Default Re: HPLC analysis of sucrose

Hello,
it might be very late reply, but I need somebody's help. Do anyone have any procedure for analyzing sugars from cyanobacteria. I have the procedure for sugar analysis by HPLC but not sugar extraction do help me.
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Old 04-17-2012, 01:14 PM
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Default Re: HPLC analysis of sucrose

Hi!
I'm trying to make the HPLC conditions including mobile phase composition, flow rate, column and Ms parameters to determinate of sucrose by HPLC-Ms. Can you help me, waiting your help!
Thankyou very much!
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