HPLC analysis of sucrose

[mironabil]

Hello, I'm trying to develop a method for sucrose analysis (just identity and not quantification) and I know reverse phase is not one of the options, so what is recommended; normal phase HPLC? and is it only refractive index detectors that can be used? no way to use UV?vis detector? Awaiting your help

[wadie]

thanks

[shelby]

Dionex Ion Chromotography with pH detection. CarboPac column. They should be able to help you. I used it for quantification, but identity is easier

[wadie]

very very interestating

[wadie]

very very good

[latha_damu]

I am having shimadzu Reverse Phase HPLC with RI detector and ODS C18 column. Can I estimate sucrose in peanut seeds with the above equipment. kindly give any protocol pertaining to this.

[helan]

Hi,
In an HPLC analysis procedure, a small volume of the sample is introduced to the mobile phase stream; the pace of progress of the analyte through the column will be quicker or slower depending on the physical and/or chemical interactions between the analyte and the stationary phase as the sample travels through the column.

As stated above, the time between introduction of the sample and elution is referred to as the retention time; this time provides a relatively unique characteristic of the analyte which is useful in identification of the compounds contained in the sample.

Thanks.

[dinesh2dg]

Hello,
it might be very late reply, but I need somebody's help. Do anyone have any procedure for analyzing sugars from cyanobacteria. I have the procedure for sugar analysis by HPLC but not sugar extraction do help me.

[nguyenunglong]

Hi!
I'm trying to make the HPLC conditions including mobile phase composition, flow rate, column and Ms parameters to determinate of sucrose by HPLC-Ms. Can you help me, waiting your help!
Thankyou very much!