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| Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques. |
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#1
09-20-2006, 07:02 PM
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 HPLC for the purification of proteins What is the best strategy to purify an unknown protein using HPLC?  |
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#2
01-29-2007, 10:07 PM
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| Re: HPLC for the purification of proteins uv detection at 280 nm, use a c18 column, use a fraction collector and collect by peak fractionation.. |
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#3
02-01-2007, 05:20 AM
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| Re: HPLC for the purification of proteins Awesome! thanks MicroKiller! |
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#5
09-28-2008, 02:27 PM
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| Re: HPLC for the purification of proteins Quote:
instead of using 280nm, can cawhy dont we use PDA to screen a range of different wavelength then for fractions colelction? we wont miss any that might not be/ less active under a that particular wavelength ? correct me if i making wrong statement~~thanks |
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| hplc , proteins , purification |
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