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-   -   HPLC for the purification of proteins (http://www.molecularstation.com/forum/chromatography-forum/424-hplc-purification-proteins.html)

moleculardude 09-20-2006 07:02 PM

HPLC for the purification of proteins
 
What is the best strategy to purify an unknown protein using HPLC?

MicroKiller 01-29-2007 11:07 PM

Re: HPLC for the purification of proteins
 
uv detection at 280 nm, use a c18 column, use a fraction collector and collect by peak fractionation..

moleculardude 02-01-2007 06:20 AM

Re: HPLC for the purification of proteins
 
Awesome!

thanks MicroKiller!

serginho 12-11-2007 12:27 PM

Re: HPLC for the purification of proteins
 
hello nice help

marcus^^ 09-28-2008 02:27 PM

Re: HPLC for the purification of proteins
 
Quote:

Originally Posted by MicroKiller (Post 2075)
uv detection at 280 nm, use a c18 column, use a fraction collector and collect by peak fractionation..

sorry...i have another opinion~~
instead of using 280nm, can cawhy dont we use PDA to screen a range of different wavelength then for fractions colelction? we wont miss any that might not be/ less active under a that particular wavelength ?

correct me if i making wrong statement~~thanks


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