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moleculardude

09-20-2006 07:02 PM

HPLC for the purification of proteins

What is the best strategy to purify an unknown protein using HPLC?

MicroKiller

01-29-2007 10:07 PM

Re: HPLC for the purification of proteins

uv detection at 280 nm, use a c18 column, use a fraction collector and collect by peak fractionation..

moleculardude

02-01-2007 05:20 AM

Re: HPLC for the purification of proteins

Awesome!

thanks MicroKiller!

serginho

12-11-2007 11:27 AM

Re: HPLC for the purification of proteins

hello nice help

marcus^^

09-28-2008 02:27 PM

Re: HPLC for the purification of proteins

Quote:

Originally Posted by MicroKiller (Post 2075)

uv detection at 280 nm, use a c18 column, use a fraction collector and collect by peak fractionation..

sorry...i have another opinion~~
instead of using 280nm, can cawhy dont we use PDA to screen a range of different wavelength then for fractions colelction? we wont miss any that might not be/ less active under a that particular wavelength ?

correct me if i making wrong statement~~thanks

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