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Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.

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HPLC for the purification of proteins

Chromatography Forum

Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.



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  #1 (permalink)  
Old 09-20-2006, 07:02 PM
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Default HPLC for the purification of proteins

What is the best strategy to purify an unknown protein using HPLC?
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  #2 (permalink)  
Old 01-29-2007, 10:07 PM
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Default Re: HPLC for the purification of proteins

uv detection at 280 nm, use a c18 column, use a fraction collector and collect by peak fractionation..
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Old 02-01-2007, 05:20 AM
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Default Re: HPLC for the purification of proteins

Awesome!

thanks MicroKiller!
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Old 12-11-2007, 11:27 AM
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Default Re: HPLC for the purification of proteins

hello nice help
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