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Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


Immunoaffinity Chromatography

Immunoaffinity Chromatography - Chromatography Forum

Immunoaffinity Chromatography - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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  #1  
Old 07-23-2008, 12:04 PM
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Default Immunoaffinity Chromatography



Hello,
please I need a method or protocol for the coupling of antibody to a matrix.

I need to prepare some columns for immunoaffinity chromatography.

thanks again
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Old 07-23-2008, 08:29 PM
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Default Re: Immunoaffinity Chromatography

Do you prefer to do it hard way or the easy way.

Hard way...
See the book Immobilized Affinity Ligand Techniques by Hermanson, Mallia, Smith from Academic Press. There are protocols for attaching just about everything to something else.

The Easy way...
Get an activated support such as AminoLink Plus.
[Only registered users see links. ]
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Old 11-29-2010, 08:48 AM
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Default Re: Immunoaffinity Chromatography

i need complete protocol for protein estimation through lowry method
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