Please, is there anyone which could help me with the HPLC technique? I am new, too new at this. In few words: I do not have any idea.
The problem is that my lab have bought one about one month before than I started my work, the person who attended the couse and was trained for its use went away before I arrived without transmitting his knowledge to anyone. So the chief of the department told me to get work the HPLC. I am afraid to use it since I am not able to, but he expects that I can. He thinks that an HPLC is just like a microwave or an homevideo (he is not a scientific of course) and that I can do everything with only the instruction of the software. He doesn't accept to have spent a lot of money for the instrument and the training and that know it is in the lab to catch dust.
The HPLC is a Jasco instrument.