Spherisurb SAX column problem

[iamnotdeadyet]

Hello,

I am utilising the following column and I'm experiencing problems with it:

Spherisurb SAX 5 um 250x4,6.

I presume the column is okay, and the washing method is not.

Can anyone help me out by telling me what washing method to use?

Thanks a bunch,

RRP.

[virtu]

Hi,
What kind of "molecules" you want to strip?Cations of metal, organic hydrophobic and hydrophilic molecules, organic cations?

[pkennedy49009]

Here is the Phenomenex recommended column washing procedure for IEX columns:

Rinse w/ 10 column volumes (CV) each of:
500mM phosphate buffer pH7
10% acetic acid (aq)
5 CV of water
10 CV of phosphate buffer pH7
5 CV H2O
10 CV MeOH
10 CV H2O
For protein removal repeat above with exception:
replace 10 CV MeOH wash with 10 Cv 5M Urea or guanidine thiocyanate

In our lab we use a stripped down procedure which seems to work OK when analyzing simple mixtures -
10 CV phosphate buffer pH7
10 CV H2O
10 CV MeOH (for storage)

Hope this helps.

Paul K