| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
05-26-2007, 08:43 AM
| |||||||||||
| |||||||||||
 Trouble shooting in HPLC We are vaidating a HPLC UV-VIS detector. We find a very peculiar problem. We found that when repeatible values on a single sample are recorded, the first time, we find the correct absorbance. This value falls for subsequent injections. Further there is a gradual decrease on further injections. We tried to see where the problem, we donot find any problem with the hardware of the detector. The tubes going to the flow cell are quite fine. The same setup with a different detector is working quite fine. We doubted the flow cell, the problem persists even on changing the flow. Does someone has an answer for this. We will be very thankful to you if you can guide us through the problem for a solution.  |
|
| Tags |
| hplc , shooting , trouble |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| ames test trouble shooting | gopi2989 | Protocols and Methods Forum | 0 | 04-01-2010 03:22 AM |
| Trouble shooting with 15% Gel | Jyona | SDS-PAGE Gel Electrophoresis Forum | 6 | 07-06-2009 03:48 PM |
| help for trouble shooting in 2-D | irfan_sadiq | 2-D Gel Electrophoresis Forum | 2 | 02-15-2009 04:19 PM |
| Trouble shooting western blott | pintu | Basic Lab Protocols and Techniques | 1 | 08-15-2006 10:38 PM |
| Yeast two hybrid trouble shooting.. | Yeast Forum | 0 | 03-22-2006 08:26 PM | |
Linear Mode
