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Chromatography Forum Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


HPLC theory!

HPLC theory! - Chromatography Forum

HPLC theory! - Discuss Chromatography techniques in this forum. Includes a HPLC forum, and other "liquid phase" separation techniques.


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Old 03-25-2007, 07:51 PM
Pipette Filler
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Default HPLC theory!



Please, help me with some theory in HPLC!
How the concentration of buffer influence on the retention time of the analyte? In my case when I change the concentration of KH2PO4 buffer from 0.05M to 0.01M (pH=7.4), the retention time of the analysed substance decrease from 15 min to 18 min.
I need to explain this fact
I think, that increasing of buffer's concentration leads to growing of the ionic power of the solution,
but what is the result from this?

Thank you!
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