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03-25-2007, 07:51 PM
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 HPLC theory! Please, help me with some theory in HPLC! How the concentration of buffer influence on the retention time of the analyte? In my case when I change the concentration of KH2PO4 buffer from 0.05M to 0.01M (pH=7.4), the retention time of the analysed substance decrease from 15 min to 18 min. I need to explain this fact  I think, that increasing of buffer's concentration leads to growing of the ionic power of the solution, but what is the result from this? Thank you!        |
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