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#1
01-24-2013, 06:17 PM
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 Several bands after cDNA PCR Hello, I have performed a PCR on a genomic sequence and after electrophoresis I obtained just one band. However, when I performed a PCR on cDNA with the same primers I obtained 2 or three bands, depending on the sample. How can I know if such different bands result from alternative splicing of the mRNA or if it is just an artifact due to non-optimal PCR conditions?  |
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#2
01-25-2013, 12:07 AM
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 Re: Several bands after cDNA PCR For PCR related issues should post here [Only registered users see links. ] Can you tell us what is the difference in size of these bands? |
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admin (02-17-2013)
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#3
01-26-2013, 04:05 AM
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| Re: Several bands after cDNA PCR very nice post. |
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| alternative splicing , bands , cdna , pcr |
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