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Several bands after cDNA PCR

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Old 01-24-2013, 07:17 PM
Pipette Filler
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Red face Several bands after cDNA PCR



Hello,

I have performed a PCR on a genomic sequence and after electrophoresis I obtained just one band. However, when I performed a PCR on cDNA with the same primers I obtained 2 or three bands, depending on the sample. How can I know if such different bands result from alternative splicing of the mRNA or if it is just an artifact due to non-optimal PCR conditions?
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Old 01-25-2013, 01:07 AM
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Default Re: Several bands after cDNA PCR

For PCR related issues should post here [Only registered users see links. ]

Can you tell us what is the difference in size of these bands?
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Old 01-26-2013, 05:05 AM
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Default Re: Several bands after cDNA PCR

very nice post.
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alternative splicing , bands , cdna , pcr


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