Several bands after cDNA PCR
I have performed a PCR on a genomic sequence and after electrophoresis I obtained just one band. However, when I performed a PCR on cDNA with the same primers I obtained 2 or three bands, depending on the sample. How can I know if such different bands result from alternative splicing of the mRNA or if it is just an artifact due to non-optimal PCR conditions?
Re: Several bands after cDNA PCR
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Can you tell us what is the difference in size of these bands?
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